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Image Search Results
Journal: Cerebral cortex (New York, N.Y. : 1991)
Article Title: Stage-specific modulation of cortical neuronal development by Mmu-miR-134.
doi: 10.1093/cercor/bhq262
Figure Lengend Snippet: Figure 3. miR-134 expression is observed in the E13.5 CP. miR-134 expression was observed predominantly in the CP. The majority of Dcx immune-positive cells in the outermost MZ (A) were (B) miR-134 negative as shown by confocal Z stack (C). Arrowheads denote Dcxþ miR-134 cells in the MZ. Arrrows denote Dcx miR-134þ cells. In contrast, miR-16 probes (E, green) were detected in Dcxþ cells of the MZ (D, green) and Nestin-positive cells of the VZ/SVZ (E, red) in addition to CP cells. Confocal Z stack demonstrated consistent overlap of Dcx and Nestin with miR-16 throughout the cortical neuroepithelium (F) in contrast to miR-134. (G, H) LSC microscope Z-stack image of an IZ neuron (asterisk/boxed region in C) subjected to RNA FISH for miR-134 (green) and then immune stained for (G) Dcx (red, top panel) and (H) Nestin (red, bottom panel). The Dcxþ
Article Snippet: Themembrane was probed with specific antibodies to Chrdl-1 protein (1:500, goat immunoglobulin G [IgG], R&D Systems, BAF-1080), Dcx protein (1:1000, goat IgG; Santa Cruz Biotechnology, sc8066),
Techniques: Expressing, Microscopy, Staining